LC/MS Characterization of Flavonoid Compounds From Fruits of Vitex Agnus-Castus Cultivated in Baghdad-Iraq

Abstract

Objective: the study was conducted to evaluate the qualitative and quantitative properties of flavonoid compounds from the fruits of Vitex Agnus cultivated in Baghdad-Iraq. Methods: plant fruits were collected from two famous spots at different sporadic regions in Baghdad where the plant famously grows. Powered plant fruit was extracted with 95% ethanol. The extract was filtered, and then evaporated to dryness using rotary evaporator. Results: The LC‐chromatogram obtained from the fruit of Vitex Agnus represented the peaks which were labeled according to the order of their retention time. The highest peaks of the major compounds belong to flavonoids; vitexin, quercetin, luteolin, taxifolin, eupatilin, iso-orientin and baicalien. Conclusion: The percentage of flavonoid compounds of vitex agnus fruits grown in Baghdad soil was found to be satisfactorily abundant and only slightly lower than humid region when compared to semi-arid regions like Baghdad.