Gstp1 Gene Polymorphism and Oxidative Stress in Coronary Artery Disease

Abstract

Objectives: The primary purpose of this study is to analyze single nucleotide polymorphism (SNP)  rs1695 of GSTP1 and oxidative stress in Coronary artery disease (CAD) and its association with CAD. Experimental Design: The study involved 50 CAD subjects as a case and 30 healthy subjects as control. Venous blood was drawn to analyze oxidative stress markers and antioxidants. For the genetic analysis of SNPs of GSTP1, genomic DNA was extracted, amplified by a polymerized chain reaction and detected by sequencing reaction. Result: Conventional risk factors, elevated  MDA levels, and low levels of antioxidants were significantly (p <0.000) associated with CAD. Conclusion: Present study could not observe a significant association of GSTP1 gene SNP with CAD and traditional risk components of CAD. However, there was no significant difference in allele (A and G) frequencies, and genotype ('AA',' AG' and 'GG') frequencies between cases and control groups studied. Present data of the South-Indian Tamil population agrees with the allele and genotype frequencies of the other South Asian people.