Comparison effect of glucose and mannitol concentration on cell viability of acute T- cell leukemia

Abstract

The importance of present study to compare between the effect of different levels (concentrations) of glucose and mannitol on jurkat cell viability. The cells were cultured in NG (2 g/L glucose) RPMI¬1640 medium, then the medium was removed and replaced by medium supplemented with glucose and mannitol separately (final concentrations in the medium was  4.5 and 6.5 g/L). Cells cultured in NG medium and without mannitol were used as controls.  In this study, we assessed the cell metabolic activity by measuring the capacity of mitochondrial succinate dehydrogenase to reduce the MTT to formazan in Jurkat cells. After exposure to hyperglycemic medium (4.5 and 6.5 g/L glucose) significant increases of jurkat cell metabolism by 0.3 and 0.7 fold respectively after 24 h and by 0.8 and 0.9 fold respectively after 48 h compared with ones grown in NG medium were noticed. On other hand, After exposure to 4.5 and 6.5 g/L of mannitol insignificant increases of jurkat cell metabolism by 0.2 and 0.3 fold respectively after 24 h and by 0.4 and 0.7 fold respectively after 48 h compared with ones grown in NG medium and in a lesser extent than the glucose levels. We concluded that both glucose and mannitol affected by increasing the viability of jurkat cell in different concentration and this increasing was a dose dependent manner, also the effect of glucose concentrations was significantly more than same concentrations of mannitol.