An Advanced Enrichment study of Gold Nanoparticles against the Bacterial Pathogen E.coli


  • Gargibala Satpathy, E. Manikandan


Surface-enhanced Raman scattering (SERS) is a useful tool for label-free analysis of bacterial cell.In this current research, for study of sensitive, specific and reproducible Raman spectra, we optimized the methods and concentration of gold nanoparticles (AuNPs). To optimize the SERS enhancement method, the centrifugal force, method for preparing AgNPs, concentration of AuNPs, ionic strength of the solution used to suspend the cells and density of the cells were chosen as impact factors and optimized through orthogonal experiments. Finally, the improved method could generate sensitive and reproducible SERS spectra from single Escherichia coli cells, and the SERS signals primarily arose from the cell envelope.