The Effect of the Tannin Purified from Seeds of Rhus coriaria L. on the Efflux Pump (RamA) Gene Salmonella Typhimurium


  • Prof. Dr. Essam Fadel Al-Jumaili, Teba Thaer Jwad Alawsy, Rafal J. Obeed, Ali Abdulmueen AL-lawi


gene (RamA), S. typhimurium, gene expression, Tannin,


The aim of this study is to measure the gene expression of efflux pump gene (RamA) was conducted by using real-time quantitative PCR assay of salmonella typhimurium bacteria treated with minimal inhibitory concentration (MIC) of tannin from. The results of PCR reaction showed the presence of  RamA gene in Salmonella typhimurium bacteria and this confirmed the role of this gene in the identification of S.typhimurium species and its intrinsic in this species.  The gene expression of RamA gene was conducted by using real-time quantitative PCR assay. It was found that the value of gene expression fold to RamA gene was recorded (0.00007) in the S. typhimurium, in contrast with tannin untreated samples that which was (1.0). It was obvious there was a direct proportion between MICs values and folds of gene expression, therefore the increasing of tannin concentration in the growth medium leads to increasing of gene expression. 16S rRNA gene expression results, which was used as reference gene, demonstrated that this gene was well suited as housekeeping gene because of the minimal variations of expression of this gene whether in tannin treated and untreated samples.