Antimicrobial susceptibility and molecular identification of Neisseria gonorrhoeae isolated from women's with endocervical infection

Abstract

Neisseria gonorrhoeae is the causative agent of gonorrhea, a sexually transmitted disease) STD) with high incidence worldwide gonorrhea in women is largely undiagnosed, under-reported, and untreated. The goals of this study are determination the prevalence of Neisseria gnorrhoeae in women with endocervical infection in addition to determination the antimicrobial resistance patterns of these isolates. Clinically suspected women (250) with signs and symptoms of bacterial vaginosis; enrolled in this cross-sectional investigation. Two Dacron® swab from each patient were used, the first swab was inoculated directly into Amies medium, then subcultured on chocolate and Thayer martin agar under CO2 condition (Candle jar) at 37 C for 24-48 hours, then positive cultures were subjected to further identification methods The second one placed in phosphate-buffered saline (PBS) tube, then used for molecular study.  Out of 250 endocervical swabs; 11 (4.40%) were culture positive on Thayer martin agar while PCR targeting reduction modifiable protein gene (rmp gene) revealed this gene was present in 32 (12.8%) with a product size of approximated 267 bp. The result showed that N. gonorrhoeae isolates were completely resistant to Cephalexin, Gentamicin and Trimethoprim, Rifampicin; high rate of resistance to cefotaxime (90.9%); azithromycin and Ciprofloxacin (72.7%) with (63.6%) to Ceftriaxone; moderate to low rate of resistance to amikacin; Aztreonam; imipenem (45.5%). The result showed that N. gonorrhoeae isolates had a high rate of sensitivity to Ticarcillin (81.8%) and levofloxacin (63.6%).