Transfer of plasmids with resistance to antibiotics in Enterococcus species
Keywords:Salah Al-din governorate, Antibiotic resistance, Plasmids, Enterococci
In the governorate of Salah Al-din, the sea coast suffers a strong impact of anthropic origin due to the large discharges from the drains, without prior treatment. In this way, these effluents transport intestinal microorganisms, such as those belonging to the genus Enterococcus, towards the marine environment, carrying genetic information of resistance to antibiotics, and these can be transferred horizontally to the autochthonous and alien flora of the coastal areas, allowing this This information is maintained in the environment, which can then be introduced to the human population through the consumption of hydro biological products. For this reason, and because Enterococcus is considered an ideal indicator of faecal contamination in marine environments, the study of this microorganism was important. The results of the present work allow us to affirm that Enterococcus spp. are found in the coastal waters of Salah Al-din governorate associated with human diseases, which have plasmids and possibly also other transferable elements with genetic information for antibiotic resistance, which can be transmitted horizontally to other microorganisms with which they share the habitat. The resistance observed for Enterococcus spp from marine environments allows us to presume the importance of this opportunistic pathogen in the ecosystem, especially if we consider that the species studied are associated with human infections and due to the enormous capacity reported for the genetic transfer of this information, mainly due to conjugation to other organisms. This article carried out a study of 31 strains of the Enterococcus genus obtained from Salah Al-din Coastal Areas. Using the disc diffusion method, antimicrobial resistance to 12 different clinical antibiotics was shown for the majority, and streptomycin was observed for most strains. Whether these residues in plasmid were tested using a 0.003 percent SDS cure test. Plasmide resistant to erythromycin, streptomycin, ciprofloxacin and norfloxacin strains were selected in a standard strain by conjugation and conversion tests where eight Enterococcous strains were transported to erythromycin (one strain) and streptomycin (7 strains) with frequency by their resistance plasmids.