S.aureus Antibacterial Activity of Nanocomposites of Cobalt and nickel with extracts of Aleo vera, Curcuma longa , Crocus sativus L , Cinnamomum verum and Zingiber officinale In vetro and In vivo.

Abstract

In this study, 128 samples were taken with a positive result in laboratory culture. The isolates were diagnosed based on physiological and biochemical tests in the hospital laboratory. The bacterial isolates were Staphylococcus aureus; the percentage of bacteria was (28.12%). The results showed that the sensitivity to microorganisms with the Vitek2 compact system that cause skin diseases indicated that S.aureus was the most resistant to antibiotics: Ampicillin, Ciprofloxacin, Tetracycline, Daptomycin, Erythromycin, Chloramphenicol, Vancomycin Cefixime. The plant extract with the cobalt oxide nanomaterial and nickel oxide prepared by the second method (Green synthesis) nanoparticles by plant extract of the plants Aloe vera water extract, Crocus sativus  (Crocus sativus L., Curcuma longa Curcuma longa, Zingiber officinale Zingiber officinale and Cinnamomum verum) is more efficient than the nanoparticles prepared by the first method (Artificial nanoparticle) in inhibiting the most antibiotic-resistant bacteria isolates of S.aureus. The solutions were at a concentration of 0.1%M. Cobalt oxide and the Aloe vera is more efficient than the extract in inhibiting S.aureus isolates with an inhibition area of ​​(45) mm in diameter. The solution of nickel oxide with Crocus sativus L. and a solution of nickel oxide with Curcuma longa is more effective in inhibiting growth with an inhibition area of ​​(43) mm diameter than the effect of a solution of nickel oxide with Zingiber officinale and nickel oxide in inhibiting isolates of Gram-positive bacteria S.aureus. Nickel oxide with Zingiber officinale had a weak effect on inhibiting bacterial growth. Plant extracts contain the following phytochemical analysis active ingredients: alkaloids, glycosides, phenols, tannins and saponins. The plant extract (Nanocomposites cobalt and nickel) succeeded in treating infections caused by S.aureus bacteria with cobalt oxide solution with aloe vera combined with nickel oxide solution and Crocus sativus L on the first day of treatment with a percentage of 0.07% M, which is the amount of MIC on testing animals Invivo. The amount of MBC for nano solutions was 0.1% M. The tests were carried out for the nano-solutions of cobalt oxide and nickel oxide prepared by the first method, and the second method was: XRD, SEM, TEM, UV, FRIT. The nanomaterials with a smaller size of 5-12nm is called quantum dot, the purest ZETA POTENTIAL(ZP ) examination of nanosolutions did not occur in it.  There was the MTT, cytotoxicity assay for nanocomposites cobalt and nickel solutions used in the treatment of mice animals notes the IC50 percentage of PC3 cancer cells is 416.8 while its value for normal HdFN cells is 101.6. The difference between the two values ​​is very large, indicating that the limits of killing cancer cells do not exceed the concentration of the substance at 416.8 and below. The normal cell limits of cell killing are at a concentration of 101.6, and the substance is not toxic to the solution to treat infections caused by S.aureus bacteria.