In vitro maturation of sheep oocytes using of Moringa Olivera leaf aqueous extract
Keywords:
oocytes, Moringa Olivera, aqueous extractAbstract
This study was conducted in the Graduate Studies Laboratory/Department of Animal Production, College of Agriculture, Al-Muthanna University for the period from November 1, 2020 to August 1, 2021, with the aim of knowing the effects of adding Moringa Olivera leaf aqueous extract (MOLAE) to the culture medium for of laboratory maturation of sheep oocytes. The MOLAE was added in concentrations of 0% (control), 5% (T1), 10% (T2), to the culture medium of in vitro maturation (RBMI-1640) of sheep oocytes with and without the cumulus cells. It was observed a significant superiority (P≤ 0.05) in the percentage of mature oocytes in the T2(55.51± 1.69) as compared with the control (45.57± 2.67) and T1 (47.14±3.14). On the other hand, there were no significant differences (P≤ 0.05) between the percentages of immature oocytes among the three treatments (control, T1, T2). The result shows a significant effect (P≤0.05) of the interaction between the concentrations of MOLAE and the presence of cumulus cells to the percentage of IVM, a highest percentage was showed in the T2 (10% MOLAE) to the sheep oocytes with cumulus cells was 55.51± 1.69 whereas the lowest percentage was showed in the T1 (5% MOLAE) to the sheep oocytes without cumulus cells was 38.67± 2.98.